strobe tuning tip!
let me preface this by saying i have been tuning my 7-string jackson (floyd bridge) with absolutely 0 issues since the day 6.0 was loaded on my ii. i see it lock dead on and even stop, with and without blocking the trem. this is with axe-edit running on a fairly slow computer (but not the mfc yet cause i haven't updated. i'm such a deadbeat.)
please take the following points as (hopefully) general unbiased information, as well as advice to take a deep breath when you tune carefully. i'm not trying to be condescending and i hope this can help you. if it doesn't, let me know.
now, to all who are having tuner issues, are you familiar with strobe-type tuning? it's a very different animal from the ubiquitous needle tuner or polytuner. shasha and several others have already alluded to this. sometimes people don't like it for this reason, but i believe the accuracy speaks for itself. on low strings especially (or even all strings), you must use a subtler approach instead of just kerranging the strings in full pick/pluck. because the tuner is so sensitive, striking it in the normal way makes the tuner go haywire. there may be a video out there from peterson about how to use a strobe tuner, but youtube is blocked here at work. here's my approach that is consistent with my peterson strobe tuner results (which i probably don't need any more!). i forgot where i found this approach from, but it is very successful.
1. like you are palm muting, slightly dampen the string so it has somewhat of a dead ring. you really only need to dampen the string you're tuning plus maybe the one above and below.
2. pick the string once (or more as necessary but not too often) almost as if you were doing a pinch harmonic, i.e. with the flat of your thumb and the pick at a slight angle from perpendicular to the string
3. let the note ring a bit and watch the "8 ball" rotate till it stops as you tune slowly up (or down then back up)
4. check again with same dampened strike, adjust as necessary
5. repeat as necessary for all strings
6. turn tho vol and/or tone knobs down a tad to keep it behaving if it's still wild. i don't think i've ever had to do this, but that would help.
caveat: i am not doing this with gig background noise.
hope this works!